African trypanosomiasis is ruthlessly killing the inhabitants of the Black continent. The scientific community has challenged the terrible disease that is spreading through tsetse flies.
60 million people in Africa South of the Sahara live with the threat of death for African sleeping sickness. At a late stage of trypanosomiasis, when the parasites cross the blood-brain barrier, the patient often dies.
Scientists will save the inhabitants of the Black continent through innovative methods using biosensors for glucose control parasites of trypanosomiasis. The uniqueness of the deadly disease lies in the fact that it survives at the expense of glucose in the body of the victim, noticed the experts.
In the process of creating the cure of carotid disease, the researchers observed for the metabolism of parasites with genetically encoded biosensor of glucose that combines three proteins: blue fluorescent, yellow fluorescent and glucosaminidase.
When glucosaminidase protein interacts with glucose in the parasite, two fluorescent proteins closer together. The experts then applied a spectroscopic change of control of the ratio of fluorescence intensity between the yellow and blue proteins. Interestingly, when the proteins are far from each other, the blue light from the blue fluorescent protein is maintained. However, the convergence of the proteins, the blue light fades, and yellow, from yellow fluorescent protein, increases.
The results obtained using the biosensor, giving a new view on the process of consumption and the transport of glucose African trypanosomiasis. Scientists will save 60 million people medicine to identify molecules that violate the level of glucose in the parasite.
“Ultimately, we hope that some of the damaging glucose molecules, which we now identify, can be turned into a therapeutic agent for treatment of African sleeping sickness,” concluded Ken Christensen, Professor of chemistry, Brigham young University (USA).